International Journal of Hematology

DOI: 10.1007/s12185-010-0742-2 Pages: 5-9

Red blood cell production from immortalized progenitor cell line

1. RIKEN BioResource Center, Cell Engineering Division

Correspondence to:
Yukio Nakamura
Tel: +81-29-8369124
Fax: +81-29-8369049
Email: yukionak@brc.riken.jp

Close

Abstract

The supply of transfusable red blood cells (RBCs) is not sufficient in many countries. If immortalized erythroid progenitor cell lines able to produce transfusable RBCs in vitro were established, they would be valuable resources. However, such cell lines have not been established. We have developed a robust method to establish immortalized erythroid progenitor cell lines following the induction of hematopoietic differentiation of mouse embryonic stem (ES) cells and have established many immortalized erythroid progenitor cell lines so far. Although their precise characteristics varied among cell lines, each of these lines could differentiate in vitro into more mature erythroid cells, including enucleated RBCs. Following transplantation of these erythroid cells into mice suffering from acute anemia, the cells proliferated transiently, subsequently differentiated into functional RBCs, and significantly ameliorated the acute anemia. Considering the number of human ES cell lines that have been established so far and the number of induced pluripotent stem cell lines that will be established in future, the intensive testing of a number of these lines for establishing immortalized erythroid progenitor cell lines may allow the establishment of such cell lines similar to the mouse erythroid progenitor cell lines.

To access the full text, please Sign in

If you have institutional access, please click here

Share the Knowledge