International Journal of Hematology

DOI: 10.1007/s12185-017-2359-1 Pages: 337-344

MAGI-1 expression is decreased in several types of human T-cell leukemia cell lines, including adult T-cell leukemia

1. Niigata University Graduate School of Medical and Dental Sciences, Division of Virology

2. Niigata University Graduate School of Medical and Dental Sciences, Division of Hematology

3. University of the Ryukyu, Department of Immunology, Graduate School and Faculty of Medicine

4. Niigata University Medical and Dental Hospital, Division of Hematopoietic Stem Cell Transplantation

Correspondence to:
Masahiro Fujii
Tel: +81(25) 227-2115
Email: fujiimas@med.niigata-u.ac.jp

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Abstract

Membrane-associated guanylate kinase with inverted orientation protein 1 (MAGI-1) is a cytoplasmic scaffold protein that interacts with various signaling molecules; it negatively controls the cell growth of various types of cells and positively controls cell–cell interaction. In T cells, MAGI-1 has been shown to inhibit Akt activity through its interaction with PTEN and MEK1. In this study we found that MAGI-1 expression is decreased in multiple (9 out of 15) human T-cell leukemia cell lines, including adult T-cell leukemia (ATL), T-cell acute lymphoblastic leukemia and chronic T-cell lymphocytic leukemia. The overexpression of MAGI-1 protein in a MAGI-1-low ATL cell line reduced cellular growth. While the overexpression of MAGI-1 protein in a MAGI-1-low ATL cell line reduced the Akt and MEK activities, the knockdown of MAGI-1 in a MAGI-1-high ATL cell line augmented the Akt and MEK activities. Collectively, the findings of the present study suggest that the decreased expression of MAGI-1 in human T cells contributes to the development of several types of T-cell leukemia, partly through the stimulation of the Akt and MEK pathways.

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