International Journal of Hematology

DOI: 10.1007/s12185-019-02766-z Pages: 75-83

Recurrent intragenic exon rearrangements of SOBP and AUTS2 in non-Hodgkin B-cell lymphoma

1. Japanese Red Cross Kyoto Daiichi Hospital, Department of Hematology

2. Fukuchiyama City Hospital, Department of Hematology

3. Kyoto Prefectural University of Medicine, Division of Hematology and Oncology

4. Hiroshima University, Department of Hematology and Oncology

5. Japanese Red Cross Kyoto Daini Hospital, Department of Hematology

6. Kyorin University, Faculty of Health Science, Department of Medical Technology

7. Kyoto Prefectural University of Medicine, Center for Molecular Diagnostics and Therapeutics

Correspondence to:
Yosuke Matsumoto
Email: yosuke-m@koto.kpu-m.ac.jp

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Abstract

Expression of intragenic exon rearrangements (IERs) has reportedly been detected in both normal and cancer cells. However, there have been few reports of occurrence of these rearrangements specific to neoplasms including malignant lymphoma. In this study, we detected IERs of ten genes (NBPF8, SOBP, AUTS2, RAB21, SPATA13, ABCC4, WDR7, PHLPP1, NFATC1 and MAGED1) in non-Hodgkin B cell lymphoma (B-NHL) cell line KPUM-UH1 using a high-resolution single nucleotide polymorphism array and reverse transcription polymerase chain reaction using reversely directed divergent primers within exons involved in genomic intragenic gains followed by sequencing analysis. Among them, the IERs involved in SOBP (6q21) exon 2 and 3 and AUTS2 (7q11.22) exon 2–4 were the molecular lesions specific to tumors and were frequently detected in B-NHL samples. These IERs constitute novel genetic alterations of B-NHL, which might be associated with tumorigenesis and be useful as genetic biological markers.

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